Journal: Virologica Sinica
Article Title: Development of the reverse genetics system for viral hemorrhagic septicemia virus genotype IVa and its application in antiviral compound screening
doi: 10.1016/j.virs.2025.12.010
Figure Lengend Snippet: Antiviral activity evaluation of seven candidate compounds against rVHSV-EGFP infection. A Schematic workflow for antiviral compound screening. B–H Cytotoxicity profiles of xanthohumol, 1-docosanol, octyl gallate, 4-methoxycinnamaldehyde, rottlerin, eriodictyol, and 2-phenylethanol in FHM cells. Cells were treated with each compound at concentrations ranging from 0 to 100 μmol/L for 4 days, and cell viability was determined using the Cell Counting Kit-8 (CCK-8) assay. I–O GFP fluorescence in FHM cells infected with rVHSV-EGFP (rescued from FHM cells, MOI = 0.1) following co-incubation with each of the seven candidate compounds (xanthohumol: 10 μmol/L, 1-docosanol: 100 μmol/L, octyl gallate: 5 μmol/L, 4-methoxycinnamaldehyde: 20 μmol/L, rottlerin: 10 μmol/L, eriodictyol: 5 μmol/L, and 2-phenylethanol: 100 μmol/L), with fluorescence intensity quantified by flow cytometry. P Healthy FHM cells without infection were served as the negative control. Q The rVHSV-EGFP-infected FHM cells without compound treatment were served as the positive control. Data presented as mean ± SD. Statistical analyses were performed using Student’s t- test. P < 0.05 (∗), P < 0.01 (∗∗) and P < 0.001 (∗∗∗). Scale bar = 200 μm.
Article Snippet: Natural compounds, including xanthohumol (CAS: 6754-58-1), 1-docosanol (CAS: 661-19-8), octyl gallate (CAS: 1034-01-1), 4-methoxycinnamaldehyde (CAS: 1963-36-6), rottlerin (CAS: 82-08-6), eriodictyol (CAS: 1072-93-1), and 2-phenylethanol (CAS: 60-12-8) were purchased from MedChem Express (China) and dissolved in 100% dimethyl sulfoxide (DMSO).
Techniques: Activity Assay, Infection, Cell Counting, CCK-8 Assay, Fluorescence, Incubation, Flow Cytometry, Negative Control, Positive Control
